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golden gate vectors and plant modular cloning parts  (Addgene inc)


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    Addgene inc golden gate vectors and plant modular cloning parts
    Golden Gate Vectors And Plant Modular Cloning Parts, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/golden gate vectors and plant modular cloning parts/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    golden gate vectors and plant modular cloning parts - by Bioz Stars, 2026-05
    90/100 stars

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    Regulatory elements and coding sequences are cloned as Level 0 parts, enabling one-step assembly into transcriptional units mediated by BsaI (e.g. using the Plant MoClo Toolkit, ) and subsequently into multigene constructs. Numbers in parentheses represent catalogue number at Addgene. **For simplicity, all cassettes are shown assembled on the forward strand. However, the orientation of any cassette in the final construct can be altered by use of a reverse Level 1 acceptor. ‘+S’ = with stop codon ‘-S’ = no stop codon.

    Journal: PLoS ONE

    Article Title: Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering

    doi: 10.1371/journal.pone.0211598

    Figure Lengend Snippet: Regulatory elements and coding sequences are cloned as Level 0 parts, enabling one-step assembly into transcriptional units mediated by BsaI (e.g. using the Plant MoClo Toolkit, ) and subsequently into multigene constructs. Numbers in parentheses represent catalogue number at Addgene. **For simplicity, all cassettes are shown assembled on the forward strand. However, the orientation of any cassette in the final construct can be altered by use of a reverse Level 1 acceptor. ‘+S’ = with stop codon ‘-S’ = no stop codon.

    Article Snippet: Constructs were assembled using the Plant Modular Cloning (MoClo) plasmid toolkit [ ], a gift from Sylvestre Marrillonet (Addgene Kit # 1000000044).

    Techniques: Clone Assay, Construct